Heterobifunctional Crosslinkers

 

Product No. Name Pkg Price
 
98567











 
(AMAS)
a-Maleimidoacetic acid-NHS
[55750-61-3]; C10H8N2O6; Mr=252.18; mp 174-175 C
Spacer Arm length:  4.4 .

wpe22.gif (1274 bytes)

Ultra short, sulfhydryl and amino reactive heterobifunctional crosslinking reagent.1,2

1.  May, J. M. (1989) Biochemistry 28, 1718.
2.  Sayre, L. M., et.al. (1984) J. Med. Chem. 27, 1325.


Storage 0-4 C.

100mg











 
$137.50













43911













2-Maleimidoethyl amineTFA; 99+%
[146474-00-2]; C6H8N2O2CF3CO2H;   Mr=254.17; mp 124 C

wpe23.gif (1141 bytes)

Water soluble! 
Derivatize Carboxy terminus or glutamic acid side chains of peptides, to maleimido-peptides with EDCHCl.
Introduce maleimide group into glycoproteins via Nakane1 coupling.
Used to interchange functional groups.


1. Nakane, P.K. (1975) Ann. N.Y. Acad. Sci. 254, 203.

Storage 0-4 C.

100mg













$110.00













98321













(BMPS); (Molbio Ultra, 99+%)
3-Maleimidopropionic acid NHS
[55750-62-4]; C11H10N2O6; Mr=266.21; mp 171 C
Spacer Arm length:  6.9 .

wpe21.gif (1317 bytes)

Short, sulfhydryl and amino reactive heterobifunctional crosslinking reagent.1,2
Used to crosslink haptens to enzymes.3

Wide application in immunodiagnostics.4

1.  Kitagawa, T. (1981) Enz. Immunoassay, 81.
2.  Tanimori, H., et.al. (1983) J. Immunol. Meth. 62, 123.
3.  Hashida, S., et.al. (1984) J. Appl. Biochem. 6, 56.
4.  Moroder, L., et.al. (1983) Biopolymers 22, 481.


Storage 0-4 C
.

500mg
1.0g












$225.00
$425.00












98335










3-Maleimidopropionic acid-PFP ester; 99 %
C13H6NO4F5;   Mr=335.19; mp 133-134 C
Spacer Arm length:  6.9 .

wpe24.gif (1370 bytes)

Heterobifunctional crosslinking reagent.
Highly electrophilic leaving group enables direct labeling of cytosine.

Storage 0-4 C.

100mg










$110.00










98799












(GMBS); (Molbio Ultra, 99+%)
4-Maleimidobutyric acid NHS
[80307-12-6]; C12H12N2O6; Mr=280.24; mp  124-125 C
Spacer Arm length:  6.8 .

wpe2B.gif (1358 bytes)

Sulfhydryl and amino reactive heterobifunctional crosslinking reagent.1,2
Introduces maleimide groups into IgM.3
Used to prepare enzyme-hapen conjugates.4


1.  Keller, O., Rudinger, Z.J. (1975) Helv. Chim. Acta. 58, 531.
2.  Tanimori, H., et.al. (1983) J. Immunol. Meth. 62, 123.
3.  Fujiwara, K., et.al. (1988) J. Immunol. Meth. 112, 77-83.
4.  Moroder, L., et.al. (1983) Biopolymers 22, 481.

Storage 0-4 C.

100mg
250mg
1.0g










$110.00
$235.00
$875.00










98654











 

 

 

 

(EMCS); 99 %
6-Maleimidocaproic acid NHS, 99%
[55750-63-5]; C14H16N2O6; Mr=308.3;  mp  68-69 C
Spacer Arm length:  9.4 .

wpe28.gif (1388 bytes)

Sulfhydryl and amino reactive heterobifunctional crosslinking reagent.1
Reagent useful for crosslinking to proteins.2
Used to prepare site-specific glycosylation reagents for peptides and proteins.3

1.  Tanimori, H., et.al. (1981) J. Pharm. Dyn., 4, 812.
2.  Partis, M.D., et.al. (1983) J. Prot. Chem. 2, 263.
3.  Ni, J.; Singh, S.; Wang, L.X. (2003) Bioconjugate Chem. 14, 232-238.

Storage 0-4 C.

100mg
500mg










$137.50
$575.00










98683 LC-EMCS; 99 %
6-[N-(6-Maleimidocaproyl)]caproic acid NHS
C20H27N3O7; Mr=421.45
Spacer Arm length:  16.8 .

A C-6 chain extended analogue of EMCS.
Crosslinks amino and sulfhydryl groups.

Storage 0-4 C.

50mg $125.00
98650












(sulfo-EMCS); 99 %
6-Maleimidocaproic acid sulfo-NHS

C14H15N2O9SNa; Mr=410.33
Spacer Arm length:  9.4 .

wpe2C.gif (1458 bytes)

Water soluble!
Sulfhydryl and amino reactive heterobifunctional crosslinking reagent.1
Used to conjugate antisense morpholino oligomers to peptides.2

1.  Kitagawa, T., et.al. (1981) Chem. Pharm. Bull. 29, 1130.
2.  Moulton, H. M., Nelson, M. H., Hatlevig, S. A., Reddy, M. T., Iverson, P. L. (2004) Bioconjugate Chem. 15, 290-299.

Storage 0-4 C.

100mg 
1.0g











$170.50
$1,000.00











98655












(Mal-cap-NPSA)
6
-Maleimidocaproic acid (2-nitro-4-sulfo) phenyl ester sodium salt
C16H15N2O9SNa; Mr=434.35

Water soluble!
Quantitate reaction with amines by release of 2-nitro 4-sulfo phenol.1
Used to prepare a PEG-maleimide for pegylation of IL-2.2


1.  Aldwin, L., Nitecki, D. (1987) Anal. Biochem. 164, 494.
2.  Goodson, R. J., Katre, N. V. (1990) Bio/Technology 8, 343-346.


Storage 0-4 C.

100mg
1.0g











$125.00
$725.00











42943










(sulfo-KMUS); 99 %
11-Maleimidoundecanoic acid sulfo-NHS
C19H25N2O9SNa; Mr=485.47; mp  245 C (Decomp)
Spacer Arm length:  15.7 .

wpe2D.gif (1525 bytes)

Water soluble!  Solubility:  1 mg/ 50mL water.
Sulfhydryl and amino reactive heterobifunctional crosslinking reagent.
Used to configure bioluminescent enzyme immunoassays (BLEIA).1

1.  Seto, Y.; Abe, K.; Itoh, M.; Sawai, H. (2002) Bioconjugate Chem. 13, 303-308.

Storage 0-4 C.

50mg










$121.00










98450









(SMPH); 99 %
Succinimidyl 6-[(3-maleimido)propionamido]-hexanoate
C17H21N3O7; Mr=379.33; mp  109-111 C
Spacer Arm length:  14.3 .

wpe31.gif (1553 bytes)

Sulfhydryl and amino reactive heterobifunctional crosslinking reagent.

Storage 0-4 C.

100mg









$137.50









98495 LC-SMPH; 99 %
C23H32N4O8; Mr=492.52;  mp  127-128.4 C
Spacer Arm length:  18.8 .

Sulfhydryl and amino reactive.
Heterobifunctional crosslinking reagent with extended chain length of 20 atoms!

Storage 0-4 C.

50 mg $100.00
42777










(BMPH); 99+%
b-Maleimidopropionic acid hydrazide
C7H10N3O3CF3CO2H;   Mr=297.19; mp  116-117 C
Spacer Arm length:  8.1 .

wpe2F.gif (1225 bytes)

 

Sulfhydryl and carbonyl  reactive heterobifunctional crosslinking reagent.1
Used to prepare thiol reactive, luminescent metal chelates.2

1.  Kitagawa, T., et.al. (1981) Chem. Pharm. Bull. 29, 1130.
2.  Ge, P.; Selvin, P.R. (2003) Bioconjugate Chem. 14, 870-876.

Storage 0-4 C.

100mg










$110.00










43660










(EMCH); 99+%
e-Maleimidocaproic acid hydrazide
C10H15N3O3CF3CO2H; Mr=339.27; mp 104-105 C
Spacer Arm length:  11.8 .

wpe2E.gif (1284 bytes)

Sulfhydryl and carbonyl  reactive heterobifunctional crosslinking reagent.
Useful for preparing immunoconjugates of doxorubicin.1

1.  Trail, P.A., et.al. (1993) Science, 261, 212-215.

Storage 0-4 C.

50mg










$82.50










43665







(KMUH); 99+%
11-Maleimidoundecanoic acid hydrazide
C15H25N3O3; Mr=295.38; mp  75 C
Spacer Arm length:  19.0 .

wpe30.gif (1360 bytes)

Sulfhydryl and carbonyl  reactive heterobifunctional crosslinking reagent.
Introduces maleimide group into glycoproteins.
Used to conjugate ligands to MoAb.1

1.  Barth, R.F., et.al. (2004) Bioconjugate Chem. 15, 185-194.

Storage 0-4 C.

50mg







$93.50







41840












3-Maleimidopropionic acid; 99+%
[7423-55-4]; C7H7NO4; Mr=169.13; mp  105-107 C
Spacer Arm length:  5.9 .

wpe32.gif (1182 bytes)

Sulfhydryl  reactive heterobifunctional crosslinking reagent.1
For preparing peptide-protein conjugates.2
Used to prepare peptide tracers and peptide-protein conjugates.3
Useful in targeted protein functionalization.4


1.  Rich, D. H., Gesellchen, P. D., Tong, A., Cheung, A., Buckner, C. K. (1975) J. Med. Chem. 18, 1004-1010.
2.  Moroder, L., et.al. (1983) BioPolymers 22, 481.
3.  Moroder, L., et.al. (1987) Hoppe-Seyler 368, 855.
4.  Meredith, G. D., Wu, H. Y., Allbritton, N. L. (2004) Bioconjugate Chem. 15, 969-982.

Storage 0-4 C.

1.0g












$137.50












43651












(N-Maleoyl-GABA); 99+%
4-Maleimidobutyric acid
[57078-98-5]; C8H9NO4; Mr=183.2; mp 97-98  C

wpe2A.gif (1206 bytes)

Heterobifunctional crosslinking reagent.
Modification reagent for thiol groups in proteins.1,2
Used to conjugate bleomycin.3


1.  Rich, D. H., Gesellchen, P. D., Tong, A., Cheung, A., Buckner, C. K. (1975) J. Med. Chem. 18, 1004-1010.
2.  Griffiths, D.G. et.al. (1981) FEBS Lett. 134, 261.
3.  Fujiwara, K., Ysuna, M., (1981) Cancer Res. 41, 4121.

Storage 0-4 C.

1.0g












$137.50












43621












(EMCA); 99+%
6-Maleimidocaproic acid
[55750-53-3]; C10H13NO4; Mr=211.21; mp 88-90 C
Spacer Arm length:  9.4 .

wpe29.gif (1250 bytes)

Sulfhydryl  reactive heterobifunctional crosslinking reagent.
Widely used probe for introducing maleimide groups into biomolecules.
Probe for thiol groups in proteins.1,2

1.  Griffith, D.G., et.al. (1981) FEBS Lett. 134, 261.
2.  Keller, O., Rudinger, Z.J. (1975) Helv. Chim. Acta. 58, 531.

Storage 0-4 C.

1.0g












$137.50












42921












11-Maleimidoundecanoic acid; 99+%
C15H23NO4; Mr=281.35; mp  89-90 C
Spacer Arm length:  15.7 .

wpe27.gif (1322 bytes)

Sulfhydryl  reactive heterobifunctional crosslinking reagent.1
For preparing peptide-protein conjugates.2

1.  Rich, D. H., Gesellchen, P. D., Tong, A., Cheung, A., Buckner, C. K. (1975) J. Med. Chem. 18, 1004-1010.
2.  Moroder, L., et.al. (1983) BioPolymers 22, 481.

Storage 0-4 C.

500mg












$93.50












98244


















SMCC; (Molbio Ultra, 99+%)
[64987-85-5]; C16H18N2O6; Mr=334.33; mp  179 C
Spacer Arm length:  11.6 .

wpe33.gif (1401 bytes)

Sulfhydryl  reactive heterobifunctional crosslinking reagent.1,2
Cyclohexane ring stabilizes maleimide group.
(Stable in 0.1 M phosphate, pH 7.0, 4 C, 64 h.)2
Conjugating anti-digoxin F(ab)2 fragments to b-galactosidase.3
Conjugating hIgG to alkaline phosphatase.4
Preparing immunogens.5


1.  Maham, D.G., et.al. (1987) Anal. Biochem. 162, 163-170.
2.  Yoshitake, S., et.al. (1979) Eur. J. Biochem. 101, 395-399.
3.  Freytag, J.W., et.al. (1984) Clin. Chem. 30(9), 1494-1498.
4.  Mahan, D. E., et.al. (1987) Anal. Biochem. 162, 163-170.
5.  Peeters, J.M., et.al. (1989) J. Immunol. Meth. 120, 133-143.

Storage 0-4 C.

100mg
250mg
1.0g
















$74.80
$137.50
$395.00
















98253

















sulfo-SMCC; 99 %
[92921-24-9]; C16H17N2O9SNa; Mr=436.37
Spacer Arm length:  11.6 .

wpe34.gif (1466 bytes)

Sulfhydryl  reactive heterobifunctional crosslinking reagent.1,2
Cyclohexane ring stabilizes maleimide group.
(Stable in 0.1 M phosphate, pH 7.0, 4 C, 64 h.)2
Conjugating anti-digoxin Fab fragments to HRP via the hinge region.3
Preparation of enzyme-antibody conjugates.4
Water soluble!


1.  Maham, D.G., et.al. (1987) Anal. Biochem. 162, 163-170.
2.  Yoshitake, S., et.al. (1979) Eur. J. Biochem. 101, 395-399.

3.  Hashida, S., et.al. (1984) J. Applied Biochem. 6, 56-63.
4.  Samoszuk, M.K., et.al. (1989) Antibody Immunocon. Radiopharm. 2(1), 37-46.

Storage 0-4 C.

50mg

















$82.50

















98485















SMCC hydrazide; 99+%
C12H17N3O3CF3CO2H;   Mr=365.31; mp  >270 C

wpe35.gif (1322 bytes)

Sulfhydryl  and carbonyl reactive heterobifunctional crosslinking reagent.
Glycoprotein reactive.
Hydrazide group reacts with aldehydes to form stable hydrazone linkage.
1

Cyclohexane ring stabilizes maleimide group.
(Stable in 0.1 M phosphate, pH 7.0, 4 C, 64 h.)2


1.  Chamow, S. M., et.al. (1992) J. Biol. Chem. 267(22), 15916-15922.
2.  Yoshitake, S., et.al. (1979) Eur. J. Biochem. 101, 395-399.

Storage 0-4 C.

100mg















$137.50















97248














LC-SMCC; 99 %
C22H29N3O7; Mr=447.44; mp 168-169 C
Spacer Arm length:  16.1 .

wpe37.gif (1626 bytes)

Sulfhydryl  and carbonyl reactive heterobifunctional crosslinking reagent.
Extended derivative of  SMCC.
Cyclohexane ring stabilizes maleimide group.
(Stable in 0.1 M phosphate, pH 7.0, 4 C, 64 h.)1


1.   Yoshitake, S., et.al. (1979) Eur. J. Biochem. 101, 395-399.

Storage 0-4 C.

100mg














$137.50














46915










(MBS-1)
m-Maleimidobenzoyl N-hydroxysuccinimide
[58626-38-3]; C15H10N2O6; Mr=314.25; mp  175-177 C
Spacer Arm length:  9.9 .

wpe31.gif (1346 bytes)

Sulfhydryl and amino reactive heterobifunctional protein crosslinking reagent.1

1.  Kitagawa, T., et.al. (1981) Chem. Pharm. Bull. 29, 1130.

Storage 0-4 C.

100mg
1.0g









$38.50
$225.00









46812









sulfo-MBS-1
C15H9N2O9SNa; Mr=416.13
Spacer Arm length:  9.9 .

wpe32.gif (1427 bytes)

Water soluble analog of MBS-1.1

1.  Aithal, H.N., et.al. (1988) J. Immunol. Meth. 112, 63-70.

Storage 0-4 C.

50mg









$95.00









46911









(MBS-2); 99 %
p-Maleimidobenzoyl N-hydroxysuccinimide
C15H10N2O6; Mr=314.25; mp 197-198 C

wpe30.gif (1393 bytes)

Sulfhydryl and amino reactive heterobifunctional protein crosslinking reagent.

Storage 0-4 C.

100mg
250mg








$49.50
$82.50








98661














(SVSB)
Succinimidyl-(4-vinylsulfonyl)benzoate
C13H11NSO6; Mr=309.29

wpe1B.gif (1377 bytes)

Amino and sulfhydryl reactive heterobifunctional crosslinker.
Hydrolytically stable vinylsulfone moiety!1,2
Unlike maleimides, michael addition to vinylsulfone does not produce stereoisomers.


1.  Morpurgo, M., et.al. (1996) Bioconjugate Chem. 7, 363-368.
2.  Masri, M.S., et.al. (1988) J. Protein Chem. 7, 49-54.

Storage 0-4 C.

50mg














$110.00














46827













(SIAB); 99 %
N-succinimidyl-(4-iodoacetyl) aminobenzoate
C13H11N2O5I; Mr=402.15; mp  199-200 C
Spacer Arm length:  10.6 .

wpe33.gif (1368 bytes)

Sulfhydryl and amino reactive heterobifunctional protein crosslinking reagent.
Conjugating antibodies to b-galactosidase.1
Used to prepare antibody-toxin conjugates.2



1.  Weltman, J.K., et.al. (1983) Biotechniques 1, 148-152.
2.  Cumber, A. J., et. al. (1985) Methods in Enzymology Vol. 112, pp. 207-224.

Storage 0-4 C.

100mg













$82.50













46824










sulfo-SIAB
C13H10N2O8INa; Mr=504.20

wpe34.gif (1448 bytes)

Sulfhydryl and amino reactive heterobifunctional protein crosslinking reagent.
Water soluble analog of SIAB.
Conjugating antibodies to b-galactosidase.1

1.  Weltman, J.K., et.al. (1983) Biotechniques 1, 148-152.

Storage 0-4 C.

50mg










$139.00










98641













(PMPI); (Molbio Ultra, 99+%)
p-Maleimidophenylisocyanate
C11H6N2O3; Mr=214.18; mp  121-123 C
Spacer Arm length:  8.7 .

Sulfhydryl and hydroxyl reactive, heterobifunctional protein crosslinking reagent.
For conjugation of b-estradiol, digitoxigenin, and vitamin B12 hydroxyl.1

1.  Palumbo, P.S., et.al. (1993) Bioconjugate Chem. 4, 212-218.

Storage 0-4 C.

100mg













$137.50













57899













(SATA); 99 %
N-succinimidyl S-acetylthioacetate
[76931-93-6]; C8H9NO5S; Mr=231.22; mp  85-86 C
Spacer Arm length:  2.8 .

wpe3B.gif (1236 bytes)

Reacts with primary amines to add protected sulfhydryls.1
Thioacetyl group can be deprotected with 0.02 M hydroxylamine hydrochloride to render free sulfhydryl.
Used to add thiol groups to rabbit polyclonal IgG.2
Used to add a thiol group to Int 8.3

1.  Duncan, R.J.S., et.al. (1983) Anal. Biochem. 132. 68-73.
2.  Hermanson, G. T. (1996) Bioconjugate Techniques, Academic Press, p.62.
3.  Johnson, J.E., et. al. (2004) Bioconjugate Chem. 15, 807-813.

Storage 0-4 C.

100mg
250mg













$75.00
$137.50













57898













(SATP); (Molbio Ultra, 99+%)
N-succinimidyl S-acetylthiopropionate
[84271-78-3]; C9H11NO5S;  Mr=245.24; mp 63-64 C
Spacer Arm length:  4.1 .

wpe3C.gif (1255 bytes)

Reacts with primary amines to add protected sulfhydryls.1
Extended analog of SATA.
Thioacetyl group can be deprotected with 0.02 M hydroxylamine hydrochloride to render free sulfhydryl.2

1.  Duncan, R.J.S., et.al. (1983) Anal. Biochem. 132. 68-73.
2.  Fuji, N., Akaji, K., Hayashi, Y., Yajima, H. (1985) Chem. Pharm. Bull. 33, 362-367.

Storage 0-4 C.

100mg













$75.00













57114










(SBAP); 99 %
Succinimidyl 3-(bromoacetamido)propionate
C9H11N2O5Br; Mr=308.19; mp  107-110.5 C
Spacer Arm length:  6.2 .

wpe3A.gif (1310 bytes)

Sulfhydryl and amino reactive heterobifunctional crosslinking reagent.
Useful for preparing cyclic peptides, peptide conjugates, and polymers.1

1.  Inman, J.K., et.al. (1991) Bioconjugate Chem. 2, 458-463.

Storage 0-4 .

100mg










$137.50










22711












(SIA); (Molbio Ultra, 99+%)
Succinimidyl iodoacetate
[39028-27-8]; C6H6NO4I;   Mr=283.02; mp  148 C
Spacer Arm length:  1.5 .

wpe39.gif (1165 bytes)

Sulfhydryl and amino reactive heterobifunctional protein crosslinking reagent.1,2
Used to functionalize nanoparticles for application as MR contrast agents.3


1.  Eur. J. Biochem. (1984) 140, 63.
2.  Rector, E.S., Schwenk, R.J., Tse, K.S., Sehon, A., Chan, H.  (1978) J. Immun. Meth. 24, 321.
2.  Wunderbaldinger, P.; Josephson, L.; Weissleder, R. (2002) Bioconjugate Chem. 13, 264-268.

Storage 0-4 C.

250mg












$137.50












22080













(SBA); (Molbio Ultra, 99+%)
Succinimidyl bromoacetate
[42014-51-7]; C6H6NO4Br; Mr=237.00; mp 115-116  C
Spacer Arm length:  1.5 .

wpe38.gif (1180 bytes)

Sulfhydryl and amino reactive heterobifunctional crosslinking reagent.1
Sulfhydryl selectivity at neutral or slightly alkaline pH is comparable to iodoacetamide, and perhaps greater than maleimide.
2
Useful in the preparation of thiol reactive, luminescent, lanthanide chelates.
3


1.  Cuatrecasas, P., et.al. (1969) J. Biol. Chem. 244, 4316.
2.  Bernatowicz, M.S., et.al. (1986) Anal. Biochem. 155, 95.
3.  Ge, P., Selvin, P. R. (2004) Bioconjugate Chem. 15, 1088-1094.

Storage 0-4 C.

1.0g













$145.00













98842













(SMPB)
Succinimidyl 4-(p-maleimidophenyl)butyrate
[79886-55-8]; C18H16N2O6; Mr=356.32; mp  130-131 C
Spacer Arm length:  14.5 .

wpe2E.gif (1483 bytes)

Sulfhydryl and amino reactive heterobifunctional crosslinking reagent.1
Crosslinking to alkaline phosphatase.2

1.  Martin, F. J., et.al. (1982) J. Biol. Chem. 257, 286-288.
2.  Teale, J.M., et.al. (1986) J. Mol. Cell Immunol. 2, 283-292.

Storage 0-4 C.

100mg













$93.50













98007











(SFB); 99 %
Succinimidyl-p-formyl-benzoate
[60444-78-2]; C12H9NO5; Mr=247.19; mp  163 C

wpe2F.gif (1259 bytes)

Amino reactive heterobifunctional crosslinking reagent.1,2
Useful derivative for conjugating angiotensin to its receptor.
3


1.  Kraehenbuhl, J.P., et.al. (1974) J. Exptl. Med. 139, 208.
2.  Tijssen, P., et.al. (1974) Viral Immunodiagnosis p. 125, Acad. Press.
3.  Galardy, R.E., et.al. (1978) J. Med. Chem. 21, 1279.

Storage 0-4 C.

100mg











$55.00











11834












(PDA); 99+%
Pyridine dithioethylamine hydrochloride
C5H11NO2S2;   Mr=217.74; mp 128-129  C

wpe2D.gif (1130 bytes)

Sulfhydryl amination reagent.1
Used to conjugate Mitomycin C to Folic acid.2
Wide use in diagnostics, and conjugation chemistry.  Water soluble!
Quantitate thiol groups by the release of 2-thiopyridone lmax=343 nm.  e=8,080 M-1 cm-1.


1.  Johnson, R.J., et.al. (1985) J. Biol. Chem. 260, 7161-7164.
2. Christopher P. Leamon, et.al. (2005) Bioconjugate Chem 16, 803-811.

Storage 0-4 C.

250mg
1.0g











$137.50
$400.00











98001












4-(N-Maleimido)benzophenone; 99+%
[92944-71-3]; C17H11NO3; Mr=277.28; mp 155-157 C

wpe13.gif (1366 bytes)

Sulfhydryl reactive photocrosslinking reagent.1,2
Generates photoactivatable conjugates from thiols.
3
Photoactivation occurs at 350 nm.


1.  Agarwal, R., et.al. (1991) J. Biol. Chem. 266, 2272.
2.  Leszyk, J., et.al. (1988) Biochemistry 27, 6983.
3.  Tao, T., et.al. (1984) Biophys. J. 45, 261.

Storage 15-25 C.

100mg
500mg











$71.50
$275.00











98002











4-(N-succinimidylcarboxy)benzophenone; 99+%
C18H13NO5; Mr=323.30; mp 205-206 C

wpe14.gif (1445 bytes)

Amino reactive photocrosslinking reagent.1
Converts amines into photoaffinity probes or surface modification reagents.2

1.  Rajasekharan, K.N., et.al. (1991) Arch. Biochem. Biophys. 288, 584.
2.  Parker, J.M.R., et.al. (1985) J. Protein Chem. 3, 479.

Storage 0-4 C.

100mg
500mg










$71.50
$275.00










98003










4-(N-iodoacetyl)benzophenone; 99+%
C15H12NO2I;  Mr=365.17

wpe2C.gif (1347 bytes)

Sulfhydryl reactive photocrosslinking reagent. 
Useful for conjugating to carbohydrates,1 or mapping myosin structure.
2
Used to crosslink chymotrypsin with unusually high efficiency (~100%).
3


1.  Hall, L.D., Yalpani, M. (1980) Carbohydrate Res. 78, C4.
2.  Lu, R.C., Wong, A. (1989) Biochemistry 28, 4826.
3.  Campbell, P., Gioannini, T.L. (1979) Photochem. Photobiol. 29, 883.

Storage 0-4 C.

100mg










$71.50










67432


















SPDP; 99+%
(N-Succinimidyl 3-[2-pyridyldithio]propionate)
[68181-17-9]; C12H12N2S2O4; Mr=312.40; mp 78-79 C
Spacer Arm length:  6.8 .

wpe1B.gif (1337 bytes)

Sulfhydryl and amino reactive heterobifunctional protein crosslinking reagent.1,2
Protein thiolation reagent.1
Tethering to artificial membranes.2
Preparing antibody-toxin conjugates.3
Hapten preparation.4
Sulfhydryl modification can be followed by release of 2-thiopyridone, lmax=343 nm.  e=8,080 M-1 cm-1.


1.  Carlsson, J., et.al. (1978) Biochem. J. 173, 723-737.
2.  Walden, P., et.al. (1986) J. Mol. Cell Immunol. 2, 191-197.
3.  Cumber, A.J., et.al. (1985) Meth. Enzymol. 112, 207-225.
4.  Gordon, R.D., (1978) PNAS 84, 308-312.

Storage 0-4 C.

100mg
250mg
1.0g
















$145.00
$325.00
$995.00
















67986












SPDP hydrazide; (PDPH) 99 %
(3-[2-Pyridyldithio]propionyl hydrazide)
C8H11N3S2O; Mr=229.32; mp 92-93 C
Spacer Arm length:  9.2 .

wpe1D.gif (1205 bytes)

 

Sulfhydryl and carbohydrate reactive derivative of SPDP.   DTT or TCEP cleavable.
Useful for preparing carbohydrate linked immunoconjugates.1
Conjugating antibodies to nerve growth factor (NGF).
2


1.  Zara, J.J., et.al. (1991) Anal. Biochem. 194, 156-162.
2.  Friden, P.M., (1993) Science 159, 373-378.

Storage 0-4 C.

50mg












$125.00












67455











LC-SPDP; 99 %
(Succinimidyl 6-[3-(2-pyridyldithio)propionamido]hexanoate)
C18H23N3S2O5; Mr=425.52; mp  82-84 C

wpe20.gif (1523 bytes)

Sulfhydryl and amino reactive heterobifunctional protein crosslinking reagent.
Extended analog of SPDP with similar properties and uses.
1


1. Carlsson, J., et.al. (1978) Biochem, J. 173, 723-737.

Storage 0-4 C.

50mg











$225.00











67475












sulfo-LC-SPDP; 99 %
(sulfo-Succinimidyl 6-[3-(2-pyridyldithio)propionamido]hexanoate)
C18H22N3S3O8Na; Mr=527.56
Spacer Arm length:  15.6 .

wpe29.gif (1598 bytes)

Sulfhydryl and amino reactive heterobifunctional protein crosslinking reagent.
Extended analog of SPDP with similar properties and uses
1

Water soluble!

1. Carlsson, J., et.al. (1978) Biochem, J. 173, 723-737.

Storage 0-4 C.

50mg












$265.00












42170









Succinimidyl-4-[2-(tert-butoxycarbonyl)hydrazino]benzoate
C16H19N3O6; Mr=349.34; mp  206-207 C

wpe2A.gif (1470 bytes)

Amino reactive heterobifunctional crosslinking reagent with latent hydrazino group.
Modification of chromatographic supports.1
Hydrazino unmasked with TFA, HCl, or formic acid.

1.  Millington, C.R., et.al. (1998) Tet Lett. 39, 7201.

Storage 0-4 C.

100mg









$110.00









42175








Succinimidyl-4-[2-(tert-butoxycarbonyl)hydrazino]acetate;  99 %
C11H17N3O6; Mr=287.27; mp 145-146 C (Decomp)

wpe2B.gif (1347 bytes)

Amino reactive heterobifunctional crosslinking reagent with latent hydrazino group.
Hydrazino unmasked with TFA, HCl, or formic acid.

Storage 0-4 C.

50mg








$82.50








22991














(Traut's Reagent); 99 %
2-Iminothiolane hydrochloride
[4781-83-3]; C4H8SNCl; Mr=137.63; mp  193-194 C

wpe28.gif (1027 bytes)

Water soluble!  Amino reactive thiolation reagent.1,2,3
An effective RNA-protein crosslinking reagent.4
Thiolation reagent for polysaccharides.
5


1.  Traut, R., et.al. (1973) Biochemistry 12, 3266.
2.  Jue, R., et.al. (1978) Biochemistry 17, 5399-5405.
3.  Kenny, J.W., et.al. (1979) Meth. Enzymol. 59, 534.
4.  Wower, L., et.al (1981) Nucleic Acids Res. 9, 4285.
5.  Alagon, C.A., et.al. (1980) Biochemistry 19, 4341.


Storage 0-4 C.

500mg














$81.00














58654

 

 

S-Acetylmercaptosuccinic anhydride; 99+ %
[6953-60-2]; C6H6SO4; Mr=174.18; mp  86-87 C

Reacts with primary amines to add protected sulfhydryls.1
Thioacetyl group is deprotected with 0.02 M hydroxylamine hydrochloride to release free sulfhydryl.


1.  Klotz, I.M.; Heiney, R.E. (1962) Arch. Biochem. Biophys. 96, 605-612.

Storage 0-4 C.

5.0g $44.75
57110









(TFCS);  99 %
6-(N-trifluoroacetyl)caproic acid NHS
C12H15N2O5F3; Mr=324.25; mp 73-74 C
Spacer Arm length:  7.7 .

wpe21.gif (1360 bytes)

Amino reactive tether with protected amino group.
Amino group unmasked at pH 7.8-8.1, phosphate buffer (usually 25 C/15-20 min.).

Storage 0-4 C.

250mg









$137.50









57118









6-(N-t-BOC)caproic acid NHS; 99 %
C15H24N2O6; Mr=328.36; mp 81-82 C

wpe22.gif (1387 bytes)

Amino reactive tether with protected amino group.
Amino group unmasked with formic acid (25 C/20 min).

Storage 0-4 C.

500mg









$82.50









57522








(SIAX); 99 %
Succinimidyl-6-(iodoacetyl)aminocaproate
[134759-23-2]; C12H17N2O5I;   Mr=396.18

wpe23.gif (1353 bytes)

Sulfhydryl and amino reactive heterobifunctional crosslinking reagent.1

1.  Brinkley, M. (1992) Bioconjugate Chem. 3, 2.

Storage 0-4 C.

50mg








$125.00








57684







(MSA); 99 %
Methyl N-succinimidyl adipate
C11H15NO6; Mr=257.24; mp  53 C
Spacer Arm length:  7.2 .

wpe24.gif (1311 bytes)

Amino reactive with latent carboxylate group.
Carboxyl unmasked at pH 9.5, phosphate buffer.

Storage 0-4 C.

500mg







$330.00







 


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