Homobifunctional NHS esters

 

Product No. Name Pkg Price
 
57841















(BSOCOES) (Molbio Ultra, 99+%)
Syn:  bis[2-(Succinimidooxycarbonyloxy)ethyl]sulfone
C14H16N2O12S; Mr=436.35; mp 158 °C
Spacer Arm length:  13.0 Å.

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Amine reactive homobifunctional crosslinking reagent.1
Used to crosslink b-endorphin to opioid receptors.2
Base cleavable; pH 11.6, 37 ºC/2 h.1
For purification of calcitonin receptors.3

1. Zarling, D.A., Watson, A., Bach, F.H., (1980) J. Immunol. 124, 913-920.
2. Howard, A.D., et.al. (1985) J. Biol. Chem. 260, 10833-18039.
3. Bouizar, Z., et.al. (1986) Eur. J. Biochem. 155, 141-147.

Storage 0-4 °C

100mg















$71.50















57903







Succinimidyloxycarbonyl-b-alanine-hydroxysuccinimide ester
C12H13N3O8; Mr=327.25;  mp  163-164 °C

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Homobifunctional NHS ester.


Storage 0-4 °C.

250mg







$110.00







57344
















(DST), (Molbio Ultra, 99+%)
Disuccinimidyl-L-tartrate
C12H12N2O10; Mr=344.23; mp 168 °C (Decomp)
Spacer Arm length:  6.4 Å.

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Amino reactive homobifunctional crosslinking reagent for protein crosslinking.
Crosslinking the cell surface receptor of CSF-2a.1
Crosslinking of cytochrome C reductase.2
Studing ATP transport in E. coli.3
Croslinking to cellular membranes.4
Cleavable by 0.015 M NaIO4, or by 0.1 % SDS, 0.02 M NaHPO4, pH 7.0/2h.2

1. Park, L. S., Friend, D., Gillis, S., Urdal, D.L., (1986) J. Biol. Chem. 261, 205-210.
2. Smith, R. J., Capaldi, R.A., Muchmore, D., Dahlquist, F., (1978) Biochem. 17, 3719-3723.
3. Bragg, P.D., Hou, C., (1980) Eur. J. Biochem. 106, 495-503.
4. Farries, T.C., Atkinson, J.P. (1989) J. Immunol. 142, 842-847.

Storage 0-4 °C.

50mg 
















$125.00
















57345







Di-sulfo-succinimidyl-L-tartrate
C12H10N2S2O16Na2;   Mr=548.34
Spacer Arm length:  6.4 Å.

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50mg







$195.00







57439 




















(DSS) (Molbio Ultra, 99+%)
Disuccinimidyl suberate
[68528-80-3]; C16H20N2O8; Mr=368.34; mp 169-170 °C
Spacer Arm length:  11.4 Å.

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Amino reactive homobifunctional crosslinking reagent for protein crosslinking.1
Widely used biochemical probe.
Conjugating hINF-g to cell surface receptors.2
Crosslinking HCG to its receptor.3
Crosslinking IL-2 to cell surfaces.4
Crosslinking CSF-1 to cell surface receptors.5
Crosslinking angiotensin II to its receptor.6

1. Tsudo, M., et.al. (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 4215-4218.
2. Novick, D., Orchansky, P., Revel, M., Rubenstein, M. (1987) J. Biol. Chem. 262, 8483-8487.
3. Rebois, R.V., Omedeo-Sale, F., Fishman, P.H., (1981) Proc. Natl. Acad. Sci. U.S.A. 78, 2086-2089.
4. Cox, G.W., Mattieson, B.J., Giardina, S.L., Varesio, L. (1990) J. Immunol. 145, 1719-1726.
5. Morgan, C.J., Stanley, E.R., (1984) Biochem. Biophys. Res. Comm. 119, 35-41.
6. Sen, I., Bull, H.G., Sutter, R.I. (1984) Proc. Natl. Acad. Sci. U.S.A. 81, 1697-1683.

Storage 0-4 °C

1.0g




















$59.00




















57424










Disuccinimidyl sebacate
C18H24N2O8; Mr=396.39; mp 150-151 °C

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Crosslinking ligand-receptor complexes of CSF-1.1
Conjugating vasoactive intestinal peptide to its receptor.2

1. Morgan, C.J., Stanley, E.R. (1984) Biochem. Biophys. Res. Commun. 119, 35-41.
2. Wood, C.L., O'Dorisio, M.S. (1985) J. Biol. Chem. 260, 1243-1247.

Storage 0-4 °C.

1.0g










$82.50










57951










(DSD)
Disuccinimidyl dodecanoate
C20H28N2O8; Mr=424.45; mp  156-157 °C

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Amino reactive homobifunctional crosslinking reagent.1
Extended 12-atom spacer.


1. Tsudo, M., et.al. (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 4215-4218.

Storage 0-4 °C.

1.0g










$82.50










57641



















(DTSP)  99 %
Dithio-bis-succinimidyl propionate (Lomant's Reagent)
[57757-57-0]; C14H16N2S2O8;   Mr=404.42; mp  128-132 °C
Spacer Arm length:  12.0 Å.

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Amino reactive, homobifunctional and reversible crosslinking reagent.1,2
Cleavable with 10-50 mM DTT, 37 ºC/30 min.
Cleavable with TCEP, pH 5.0/5 min.6
Useful for characterizing CSF-2a receptors.3
Crosslinking antigens to monoclonal antibodies.4
Identification of receptors for VIP.2
Studing glycoproteins in human RBC.5

1. Lomant, A.J., Fairbanks, G. (1976) J. Mol. Biol. 104, 243-261.
2. Laburthe, M., Breant, B., Rouyer-Fessard, C. (1984) Eur. J. Biochem. 139, 181-187.
3. Park, L.S., Friend, D., Gillis, S., Urdal, D.L. (1986) J. Biol. Chem. 261, 205-210.
4. Hamada, H., Tsuro, T. (1987) Anal. Biochem. 160, 483-488.
5. Schweizer, E., Angst, W., Lutz, H.V., (1982) Biochem. 21, 6807-6818.
6. Han, J.C., Han, G.Y. (1994) Anal. Biochem. 220, 5-10.

Storage 0-4 °C

1.0g



















$125.00



















57212










Hexadecanediol-bis-succinimidyl carbonate
C26H40N2O10; Mr=540.61

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Extended spacer arm. Cleavable!
Dimeric adducts are cleaved with 0.1 M hydroxylamine, phosphate buffer, pH 8.1.

Storage 0-4 °C

100mg










$125.00










57428



















(BS3)
bis-sulfo-succinimidyl suberate
[82436-77-9]; C16H18N2S2O14Na2; Mr=572.43; mp >300 °C
Spacer Arm length:  11.4 Å.

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Highly versatile protein crosslinking reagent.1
Water soluble and membrane impermeable due to sodium sulfate groups.2
Used to study anion exchange in human erythrocytes.3
Useful for crosslinking insulin to its receptor.4
Covalently crosslinking b-endorphin to calmodulin.5
Used for crosslinking subunits of cytochrome b559.6

1. Souza, E.D., et.al. (1988) J. Biol. Chem. 263, 3943-3951.
2. Staros, J.V. (1982) Biochem. 21, 3950-3955.
3. Staros, J.V., Kakkad, B.P., (1983) J. Membrane Biol. 74, 247-2254.
4. Waugh, S.M., DiBella, E.E., Pilch, P.F., (1989) Biochem. 28, 3448-3455.
5. Giedroc, D.P., Keravis, T.M., Staros, J.V., Ling, N., Wells, J.N., Puett, D. (1985) Biochem. 24, 1203-11.
6. Knoller, S., Shpungin, S., Pick, E. (1991) J. Biol. Chem. 266, 2795-2804.

Storage 0-4 °C

100mg



















$82.50